Transformation of Schwanniomyces occidentalis with an ADE2 gene cloned from S. occidentalis
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چکیده
منابع مشابه
Cloning of a-amylase gene from Schwanniomyces occidentalis and expression in Saccharomyces cerevisiae
The cloning of a-amylase gene of S . occidentalis and the construction of starch digestible strain of yeast, S . cerevisiae AS. 2.1364 with ethanol-tolerance and without auxotrophic markers used in fermentation industry were studied. The yeast/E . coli shuttle plasmid YCEpl partial library of S. occidentalis DNA was constructed and a-amylase gene was screened in S. cerevisiae by amylolytic acti...
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The yeast Schwanniomyces occidentalis produces a killer toxin lethal to sensitive strains of Saccharomyces cerevisiae. Killer activity is lost after pepsin and papain treatment, suggesting that the toxin is a protein. We purified the killer protein and found that it was composed of two subunits with molecular masses of approximately 7.4 and 4.9 kDa, respectively, but was not detectable with per...
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Schwanniomyces occidentalis β-fructofuranosidase (Ffase) releases β-fructose from the nonreducing ends of β-fructans and synthesizes 6-kestose and 1-kestose, both considered prebiotic fructooligosaccharides. Analyzing the amino acid sequence of this protein revealed that it includes a serine instead of a leucine at position 196, caused by a nonuniversal decoding of the unique mRNA leucine codon...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1988
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.170.12.5572-5578.1988